National Center for Biotechnology InformationU. The well is 6. Details 18 More. Chomczynski P, Sacchi N. The method was applied to a filtered groundwater sample from a geochemically reduced zone of a shallow alluvial aquifer near Rifle, CO, where multiple potential carbon sources co-occur. Rhodopseudomonas palustris HaA2 is used to outgroup root the tree. In this study, two methods that target RNA but independently provide estimates of carbon sources through either a geochemical measurement or a taxonomic assessment indicated the important role of autotrophy in a subsurface microbial community.
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Assessing sources and ages of organic matter supporting river and estuarine bacterial production: A multiple-isotope Delta C, delta C, and delta N approach.
Shallow aquifers are often characterized as oligotrophic environments in which a heterotrophic microbial lifestyle is supported by surface-derived allochthonous organic matter 1although recent studies have demonstrated the importance of chemolithoautotrophy in some aquifer environments 2 — 4. Details 16 More. Beller HR, et al. Distribution and diversity of autotrophic bacteria in groundwater systems based on the analysis of RubisCO genotypes.
Water Resources Research 47 Details 19 More. Water-saturated phenol is generally recommended for RNA extractions, as it increases yields of RNA while removing DNA 37 ; however, in this case, residual C from the phenol treatment could not be fully removed, skewing the radiocarbon signature of the RNA.
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Methyl-compound use and slow growth characterize microbial life in 2-km-deep subseafloor coal and shale beds. Laskin — Academic Press, Microbial carbon cycling in oligotrophic regional aquifers near the Tono Uranium Mine, Japan as inferred from delta C and Delta C values of in situ phospholipid fatty acids and carbon sources.
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Corolla Hybrid 1. Proceedings of the National Academy of Sciences. Nature Communications 7, However, the exact source of the DOC is not known and could be a mixture of carbon leaching from the soil horizon and buried plant material, along with the older sedimentary organic carbon.
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Zip: They reported NIP-III AQPs (Si transporters) with a GSGR selectivity filter and a . materials are available on line. Click here for additional data file.(M, zip) et Development des Plantes, Ecole Normale Supérieure Lyon, Lyon.
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RNA was chosen as the biomolecule of interest because rRNA constitutes the majority of RNA in prokaryotes, represents recently active organisms, and yields detailed taxonomic information. Campbell KM, et al.
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Figure 1. Emerson, D. Side-Impact Air Bags Proceedings of the National Academy of Sciences. References 1.
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|RNA-Seq results showing the relative abundance of the top 20 reconstructed sequences in the Rifle aquifer RNA sample, along with cumulative abundance of all sequences blue and lithoautotrophic sequences red.
Stuiver M, Polach HA. The alluvial Rifle aquifer is underlain by the impermeable Wasatch Formation at a depth of 5. Thiobacillus thiophilus sp. Side-Impact Air Bags Twenty-four RNA samples extracted from E.