The standard thermodynamic functions under reference conditions are calculated as:. D'Angelo S. Alternatively, antigens relevant to unmet vaccine needs, such as for example, P. The method of claim 1, wherein the change in conformational dynamics of the peptidogenic proteins is measured by: a. For example, a mixture of different peptidogenic proteins or polynucleotides encoding different peptidogenic proteins derived from the same starting protein can be used to mount an immune response. Additionally, multiple antigens listed in Table 2 can be used as the starting proteins to derive multiple different peptidogenic proteins to be used as a vaccine, generate an immune response, including the raising of antibodies. Job two: fit an LSD. Maecker H T et al. Other methods of predicting or empirically discovering non-surface i.
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Combinatorial immunization, in which subjects are immunized with two or more distinct antigens that have the same overall surface features i. The virus grows in Spodoptera frugiperda cells.
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NOVEL METHODS OF GENERATING ANTIBODIES Rutgers, The State University of New Jersey
Treatment with the peptidogenic proteins, the polynucleotides encoding the peptidogenic proteins, or an antibody raised to the peptidogenic protein may be continued for as long as is necessary to achieve a therapeutic response. The vaccine can be administered intramuscularly by needle and syringe.
See Carter Nature Rev.
The peptidogenic proteins can be used to induce an immune response, which can lead to the generation of antibodies and/or can be used to vaccinate a.
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Altman J D, et al. Georgiou G, et al. The mixture of polynucleotides of claim 30, wherein the polynucleotides: a.
Thus, increasing the immunogenicity of a starting protein by altering its conformational dynamics to produce numerous peptidogenic proteins which can then be simultaneously introduced into an animal will generate a robust immune response and has the potential to raise a broader repertoire of polyclonal antibodies which can be further fractionated for example, by molecularly cloning via their respective encoding mRNAs into single isolated species.
Sites that are critical for ligand-receptor binding can also be determined by structural analysis such as crystallization, nuclear magnetic resonance or photoaffinity labeling Smith et al. Vaccination can be conducted either prophylactically or therapeutically.
In preferred embodiments, for the expression of entire antibody molecules, vectors encoding both the heavy and light chains may be co-expressed in the host cell for expression of the entire immunoglobulin molecule, as detailed below.
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