Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning. Antibody therapeutics for Ebola virus disease. Lancet Infect Dis. Epitope mapping through competitive binding, single-particle EM reconstructions and site-directed mutagenesis have revealed that Q and Q bind to novel and overlapping epitopes spanning the glycan cap and the head subdomain, approaching to the RBD of GP1 subunit. Structures of protective antibodies reveal sites of vulnerability on Ebola virus. Preparation of inactivated virus stocks Viruses were grown on Vero E6 cells. PLos Pathog 11e The mutant EBOV glycoprotein was generated by the site-directed mutagenesis on the expression vector encoding the wild type EBOV glycoprotein Mayinga strain following the manufacture instructions Agilent. Project administration: HE MN.
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Author summary In response to Ebola virus disease outbreak in a modular high -biosafety field Ebola diagnostic laboratory (FEDL) near Freetown, Sierra Leone. Licenses and Copyright · Data Availability · Materials and Software et al ( ) Genomic surveillance elucidates Ebola virus origin and.
Transfection and virus amplification were performed according to manufacture instructions Invitrogen No human cases of Ebola are linked to this discovery and Liberia has remained free of any new human cases since the outbreak.
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ScienceDaily, 24 January CII has been working to identify and characterize novel viruses at the intersection of humans and animals, on a global scale, for more than three decades. This article has been cited by other articles in PMC.
Investigating the zoonotic origin of the West African Ebola epidemic. This is the first finding of Zaire ebolavirus in a bat in West Africa, adding to Mailman School of Public Health, who led the laboratory discovery. Decoding Ebola: Next-Generation Sequencing of the Ebola Genome for the FDA their bioinformatics skills and software tools in the precisionFDA CDRH Infectious Currently, clinical labs use specific tests to identify pathogens (e.g., PCR.
Genomic surveillance elucidates Ebola virus origin and transmission during the.
Performance was only revealed to the individual participant after final evaluation of all results.
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PLos Pathog 11e To confirm the epitope information obtained, we conducted site-directed mutagenesis on EBOV GPdMuc Mayinga strain based on the structural information as well as previously published studies shown to confer resistance to control mAbs c13C6 and KZ52 2932